Schedule 1 stunning followed by decapitation

The primary reason for carrying out this technique is to obtain a large volume of blood which has not been affected by anaesthetic drugs or carbon dioxide. A large volume of blood can be collected from the trunk if necessary.

Suitable for all strains, this technique should only be used in rare circumstances and with exceptional scientific justification. The ASPA limits its use to rodents of body weight 1 kg or less. The guinea pig must be determined as dead before decapitation can take place (see Section 1(4) of the ASPA).

This is a Schedule 1 method of euthanasia and should be carried out by people competent in this method. Training for stunning and decapitation should be undertaken on dead animals.

Summary

Number of samples One
Blood volume 10 - 20 ml
Equipment Suitable sharp instrument to decapitate, (e.g. guillotine or sharp scissors).
Staff resource One person is required to take the blood sample.
Other A high level of expertise is required for this technique.

Resources and references

Schedule 1 stunning followed by decapitation sampling in other animals

Click here for information on schedule 1 stunning followed by decapitation for blood sampling in the mouseClick here for information on schedule 1 stunning followed by decapitation for blood sampling in the rat

All blood sampling techniques in the guinea pig

Click here for information on blood vessel cannulation in the guinea pigClick here for information on tarsal vein sampling in the guinea pigClick here for information on saphenous vein blood sampling in the guinea pigClick here for information on abdominal/thoracic blood vessel blood sampling in the guinea pigClick here for information on cardiac puncture blood sampling in the guinea pigClick here for information on decapitation blood sampling techniques in the guinea pig