- Resources and references
- Schedule 1 stunning followed by decapitation sampling in other animals
- All blood sampling techniques in the guinea pig
The primary reason for carrying out this technique is to obtain a large volume of blood which has not been affected by anaesthetic drugs or carbon dioxide. A large volume of blood can be collected from the trunk if necessary.
Suitable for all strains, this technique should only be used in rare circumstances and with exceptional scientific justification. The ASPA limits its use to rodents of body weight 1 kg or less. The guinea pig must be determined as dead before decapitation can take place (see Section 1(4) of the ASPA).
This is a Schedule 1 method of euthanasia and should be carried out by people competent in this method. Training for stunning and decapitation should be undertaken on dead animals.
|Number of samples||One|
|Blood volume||10 - 20 ml|
|Equipment||Suitable sharp instrument to decapitate, (e.g. guillotine or sharp scissors).|
|Staff resource||One person is required to take the blood sample.|
|Other||A high level of expertise is required for this technique.|
- A good practice guide to the administration of substances and removal of blood, including routes and volumes.
- Ness RD (1999), Clinical pathology and sample collection of exotic small animals. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3), pp 591-620
- Lucas RL, Lentz KD, Hale AS (2004), Collection and preparation of blood products. Clinical Techniques in Small Animal Practice. 19(2), pp 55-62