This is a suitable method to obtain a single, large, good quality blood sample when the marmoset is under terminal anaesthesia or euthanised, as the body cavity must be opened to expose the blood vessel.
A laparotomy is performed and viscera are moved to one side to identify the abdominal vena cava. Surrounding connective tissue and fat are then removed to expose and isolate the vessel. Application of finger pressure to the blood vessel can help dilate the vessel. Depending on the size of the marmoset, up to 10 ml of blood can be collected. Care should be taken to withdraw the blood slowly to prevent the vessel collapsing.
|Number of samples||One|
|Sample volume||Up to 10 ml|
|Staff resource||Two people are required: one to take the blood sample and another to perform the anaesthesia (unless carried out on a euthanised animal).|
Resources and references
- A good practice guide to the administration of substances and removal of blood, including routes and volumes.
- Ness RD (1999), Clinical pathology and sample collection of exotic small animals, The Veterinary Clinics of North America: Exotic Animal Practice. 2(3), 591-620
- Lucas RL, Lentz KD, Hale AS (2004), Collection and preparation of blood products. Clinical Techniques in Small Animal Practice. 19(2) 55-62
- Removal of blood from laboratory animals and birds.