- Resources and references
- Blood vessel cannulation technique in other animals
- All blood sampling techniques in the mouse
Appropriate for all strains, this is a suitable technique to obtain a single, large, good quality blood sample from a euthanised mouse or a mouse under terminal anaesthesia. A sample size of 0.4 -1.0 ml can be collected depending on the size of the mouse. As the heart is not punctured, this technique can be used when it is necessary to avoid cardiac damage.
Blood is collected either from the abdominal vena cava, abdominal aorta or aortic arch which can be accessed via a laparotomy or thoracotomy in larger mice. Removal of connective tissue and application of finger pressure is necessary to dilate the vessel. Blood should be withdrawn slowly to prevent the vessel collapsing.
|Number of samples||One|
|Sample volume||Up to 1 ml|
|Staff resource||One person is required to take the sample.|
- A good practice guide to the administration of substances and removal of blood, including routes and volumes.
- McClure DE (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 565-590
- Ness RD (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 591-620
- Lucas RL, Lentz KD, Hale AS (2004) Collection and preparation of blood products. Clinical Techniques in Small Animal Practice. 19(2) 55-62
- Methods of blood collection in the mouse.
- Removal of blood from laboratory animals and birds.