Abdominal/thoracic blood vessel

Please read the general principles of blood sampling page before attempting any blood sampling procedure.

Technique

Appropriate for all strains, this is a suitable technique to obtain a single, large, good quality blood sample from a euthanised mouse or a mouse under terminal anaesthesia. A sample size of 0.4 -1.0 ml can be collected depending on the size of the mouse. As the heart is not punctured, this technique can be used when it is necessary to avoid cardiac damage.

Blood is collected either from the abdominal vena cava, abdominal aorta or aortic arch which can be accessed via a laparotomy or thoracotomy in larger mice. Removal of connective tissue and application of finger pressure is necessary to dilate the vessel. Blood should be withdrawn slowly to prevent the vessel collapsing. Correct aseptic technique should be used.

Summary

Number of samples One
Sample volume Up to 1 ml
Equipment 25G needle
Staff resource One person is required to take the sample.

Resources and references

Abdominal/thoracic blood vessel technique in other animals

Click here for information on abdominal/thoracic blood vessel blood sampling in the ratClick here for information on abdominal/thoracic blood vessel blood sampling in the guinea pig
All blood sampling techniques in the mouse

Click here for information on blood vessel cannulation in the mouse Click here for information on tail snip blood sampling in the mouse Click here for information on tail vessel microsampling in the mouse Click here for information on tail vein blood sampling in the mouse Click here for information on saphenous vein blood sampling in the mouse Click here for information on retro-orbital blood sampling techniques in the mouse  Click here for information on cardiac puncture blood sampling in the mouse Click here for information on schedule 1 stunning followed by decapitation in the mouse Click here for information on decapitation blood sampling techniques in the mouse