Cardiac puncture

Technique

Cardiac puncture is a suitable technique to obtain a single, large, good quality sample from a euthanised mouse or a mouse under deep terminal anaesthesia if coagulation parameters, a separate arterial or venous sample or cardiac histology are not required. It is appropriate for all strains of mouse.

0.1 - 1 ml of blood can be obtained depending on the size of the mouse and whether the heart is beating. Blood samples are taken from the heart, preferably the ventricle, which can be accessed either via the left side of the chest, through the diaphragm, from the top of the sternum or by performing a thoracotomy. Blood should be withdrawn slowly to prevent the heart collapsing.

Cardiac puncture should not be used if the peritoneum needs to be lavaged to harvest cells, as this technique can cause blood to escape into the peritoneal cavity.

Summary

Number of samples One
Sample volume Up to 1 ml
Equipment 23G - 25G needle
Staff resource One person is required to take the blood sample.

Resources and references

Cardiac puncture technique in other animals

Click here for information on cardiac puncture blood sampling in the rat Click here for information on cardiac puncture blood sampling in the hamster Click here for information on cardiac puncture blood sampling in the guinea pig Click here for information on cardiac puncture blood sampling in the rabbit Click here for information on cardiac puncture blood sampling in the ferret

All blood sampling techniques in the mouse

Click here for information on blood vessel cannulation in the mouse Click here for information on tail snip blood sampling in the mouse Click here for information on tail vessel microsampling in the mouse Click here for information on tail vein blood sampling in the mouse Click here for information on saphenous vein blood sampling in the mouse Click here for information on retro-orbital blood sampling techniques in the mouse Click here for information on abdominal/thoracic blood vessel blood sampling in the mouse Click here for information on schedule 1 stunning followed by decapitation in the mouse Click here for information on decapitation blood sampling techniques in the mouse