- Resources and references
- Blood vessel cannulation technique in other animals
- All blood sampling techniques in the mouse
Cardiac puncture is a suitable technique to obtain a single, large, good quality sample from a euthanised mouse or a mouse under deep terminal anaesthesia if coagulation parameters, a separate arterial or venous sample or cardiac histology are not required. It is appropriate for all strains of mouse.
0.1 - 1 ml of blood can be obtained depending on the size of the mouse and whether the heart is beating. Blood samples are taken from the heart, preferably the ventricle, which can be accessed either via the left side of the chest, through the diaphragm, from the top of the sternum or by performing a thoracotomy. Blood should be withdrawn slowly to prevent the heart collapsing.
Cardiac puncture should not be used if the peritoneum needs to be lavaged to harvest cells, as this technique can cause blood to escape into the peritoneal cavity.
|Number of samples||One|
|Sample volume||Up to 1 ml|
|Equipment||23G - 25G needle|
|Staff resource||One person is required to take the blood sample.|
- A good practice guide to the administration of substances and removal of blood, including routes and volumes.
- McClure DE (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 565-590.
- Ness RD (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 591-620.
- Lucas RL, Lentz KD, Hale AS (2004) Collection and preparation of blood products. Clinical Techniques in Small Animal Practice. 19(2) 55-62.
- Methods of blood collection in the mouse.
- Removal of blood from laboratory animals and birds.