Please read the general principles of blood sampling page before attempting any blood sampling procedure.
- Resources and references
- Retro-orbital technique in other animals
- All blood sampling techniques in the mouse
Also referred to as peri-orbital, posterior-orbital and orbital venous sinus bleeding. Retro-orbital bleeding should always be performed as a terminal procedure. Adverse effects reported for this procedure are described in the summary below.
Where its use is unavoidable, retro-orbital bleeding should only be used under general anaesthesia. Because of the severity of the adverse effects that can occur with this technique, even in skilled hands, it is essential that it is conducted only by staff members competent (practiced) in the technique.
Blood is collected from the venous sinus. The mouse is restrained, the neck gently scruffed and the eye made to bulge. A capillary tube/pipette is inserted medially, laterally or dorsally. Blood is allowed to flow by capillary action into the capillary tube/pipette, ensure proper aseptic technique is used. The sample obtained is a mixture of venous blood and tissue fluid, and is not representative of venous blood.
|Number of samples||It is recommended that only one sample be taken.|
|Sample volume||Up to 0.2 ml with recovery; Up to 0.5 ml non-recovery.|
|Equipment||A glass capillary tube or Pasteur pipette.|
|Staff resource||One person is required to take the blood sample.|
|Other||Careful monitoring for adverse effects is necessary both peri- and post-operatively.|
- A good practice guide to the administration of substances and removal of blood, including routes and volumes.
- Luzzi M et al. (2005) Collecting blood from rodents: a discussion by the Laboratory Animal Refinement and Enrichment Forum. Animal Technology and Welfare. 4(2) 99-102.
- Schnell MA, Hardy C, Hawley M, Propert KJ, Wilson JM (2002) Effect of blood collection technique in mice on clinical pathology parameters. Human Gene Therapy. 13(1) 155-161.
- Methods of blood collection in the mouse.
- Forbes N, Brayton C, Grindle S, Shepherd S, Tyler B, Guarnieri M (2010) Morbidity and mortality rates associated with serial bleeding from the superficial temporal vein in mice. Lab Animal Europe. 10(9), 14-22
- Removal of blood from laboratory animals and birds.