- Resources and references
- Blood vessel cannulation technique in other animals
- All blood sampling techniques in the mouse
The primary reason for carrying out this technique is to obtain a large volume of blood that has not been affected by anaesthetic drugs or carbon dioxide. A large volume of blood can be collected from the trunk if necessary.
Suitable for all strains, this technique should only be used in rare circumstances and where there is exceptional scientific justification. Concussion of the brain is acheived by striking the cranium. The mouse must be determined as dead before decapitation (see Section 1(4) of the amended ASPA).
Concussion of the brain is a Schedule 1 method of euthanasia and should only be carried out by people competent in this method for the species and size of the animal. Training for stunning and decapitation should be undertaken on dead animals.
|Number of samples||One|
|Sample volume||Up to 1 ml|
|Equipment||Suitable sharp instrument to decapitate, (e.g., guillotine or sharp scissors).|
|Staff resource||One person is required to take the blood sample.|
|Other||A high level of expertise is required for this technique.|
- A good practice guide to the administration of substances and removal of blood, including routes and volumes.
- McClure DE (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 565-590.
- Ness RD (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 591-620.
- Lucas RL, Lentz KD, Hale AS (2004) Collection and preparation of blood products. Clinical Techniques in Small Animal Practice. 19(2) 55-62.