Schedule 1 stunning followed by decapitation

Technique

The primary reason for carrying out this technique is to obtain a large volume of blood that has not been affected by anaesthetic drugs or carbon dioxide. A large volume of blood can be collected from the trunk if necessary.

Suitable for all strains, this technique should only be used in rare circumstances and where there is exceptional scientific justification. Concussion of the brain is acheived by striking the cranium. The mouse must be determined as dead before decapitation (see Section 1(4) of the amended ASPA).

Concussion of the brain is a Schedule 1 method of euthanasia and should only be carried out by people competent in this method for the species and size of the animal. Training for stunning and decapitation should be undertaken on dead animals.

Summary

Number of samples One
Sample volume Up to 1 ml
Equipment Suitable sharp instrument to decapitate, (e.g., guillotine or sharp scissors).
Staff resource One person is required to take the blood sample.
Other A high level of expertise is required for this technique.

Resources and references

  • A good practice guide to the administration of substances and removal of blood, including routes and volumes.
  • McClure DE (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 565-590.
  • Ness RD (1999) Clinical pathology and sample collection in the laboratory rodent. The Veterinary Clinics of North America: Exotic Animal Practice. 2(3) 591-620.
  • Lucas RL, Lentz KD, Hale AS (2004) Collection and preparation of blood products. Clinical Techniques in Small Animal Practice. 19(2) 55-62.

Schedule 1 stunning followed by decapitation technique in other animals

Click here for information on schedule 1 stunning followed by decapitation for blood sampling in the mouse Click here for information on schedule 1 stunning followed by decapitation for blood sampling in the mouse
All blood sampling techniques in the mouse

Click here for information on blood vessel cannulation in the mouse Click here for information on tail snip blood sampling in the mouse Click here for information on tail vessel microsampling in the mouse Click here for information on tail vein blood sampling in the mouse Click here for information on saphenous vein blood sampling in the mouse Click here for information on retro-orbital blood sampling techniques in the mouse Click here for information on abdominal/thoracic blood vessel blood sampling in the mouse Click here for information on cardiac puncture blood sampling in the mouse  Click here for information on decapitation blood sampling techniques in the mouse