- Resources and references
- Abdominal/thoracic blood vessel sampling in other animals
- All blood sampling techniques in the rat
Appropriate for all strains of rat, this is a suitable technique to obtain a single, large, good-quality blood sample from a euthanised rat or a rat under terminal anaesthesia. A sample size of 5-10 ml can be collected from the hepatic portal vein, or 10-15 ml from other abdominal/thoracic vessels, depending on the size of the rat. As the heart is not punctured, this technique can be used where it is necessary to avoid damage to the heart.
Blood is collected either from the abdominal aorta, caudal or dorsal aorta, vena cava or hepatic portal vein which are accessed via a laparotomy or thoracotomy. Removal of connective tissue and application of finger pressure is necessary to dilate the vessel. Blood should be withdrawn slowly to prevent the vessel collapsing. Deep surgical anaesthesia is necessary unless the rat is dead.
A video of this technique is available (hepatic portal vein) - contact the NC3Rs via firstname.lastname@example.org.
|Number of samples||One|
|Sample volume||Up to 10 ml from the hepatic portal vein, or 15 ml from other abdominal/thoracic vessels, depending on the size of the rat.|
|Equipment||19 - 21G needle|
|Staff resource||One person is required to take the sample.|