Cardiac puncture

Technique

Cardiac puncture is a suitable technique to obtain a single, large, good quality sample from a euthanised rat or a rat under deep terminal anaesthesia if coagulation parameters, a separate arterial or venous sample or cardiac histology are not required. It is appropriate for all strains.

A sample of 10 - 15 ml of blood can be obtained depending on the size of the rat and whether the heart is beating. Blood samples are taken from the heart, preferably the ventricle, which can be accessed either via the left side of the chest, through the diaphragm, from the top of the sternum or by performing a thoracotomy. Blood should be withdrawn slowly to prevent the heart collapsing.

Summary

Number of samples One
Sample volume Up to 15 ml
Equipment 19G - 21G needle
Staff resource One person is required to take the blood sample.

Resources and references

Cardiac puncture sampling in other animals

Click here for information on cardiac puncture blood sampling in the mouseClick here for information on cardiac puncture blood sampling in the hamsterClick here for information on cardiac puncture blood sampling in the guinea pigClick here for information on cardiac puncture blood sampling in the rabbitClick here for information on cardiac puncture blood sampling in the ferret

All blood sampling techniques in the rat

Click here for information on blood vessel cannulation in the ratClick here for information on tail vein blood sampling in the ratClick here for information on jugular vein blood sampling in the ratClick here for information on saphenous vein blood sampling in the ratClick here for information on retro-orbital blood sampling techniques in the ratClick here for information on using a temporary cannula for blood sampling in the ratClick here for information on abdominal/thoracic blood vessel blood sampling in the ratClick here for information on schedule 1 stunning followed by decapitation for blood sampling in the ratClick here for information on decapitation blood sampling techniques in the rat