Abdominal/ thoracic blood vessel

Technique

Appropriate for all strains, this is a suitable technique to obtain a single, large, good quality blood sample from a euthanised guinea pig or a guinea pig under terminal anaesthesia. A sample volume of 10 - 15ml can be collected depending on the size of the guinea pig. As the heart is not punctured, this technique can be used where it is necessary to avoid damage to the heart.

Blood is collected either from the abdominal aorta, caudal or dorsal aorta or vena cava via a laparotomy or thoracotomy. Removal of connective tissue and application of finger pressure may be necessary to dilate the vessel. Blood should be withdrawn slowly to prevent the vessel collapsing. Deep surgical anaesthesia is necessary unless the guinea pig is dead.

Summary

Number of samples One
Sample volume Up to 15 ml, depending on the size of the guinea pig.
Equipment 19G - 21G needle
Staff resource One person is required to take the blood sample.

Resources and references

Abdominal/thoracic sampling in other animals

Click here for information on abdominal/thoracic blood vessel blood sampling in the mouseClick here for information on abdominal/thoracic blood vessel blood sampling in the rat

All blood sampling techniques in the guinea pig

Click here for information on blood vessel cannulation in the guinea pigClick here for information on tarsal vein sampling in the guinea pigClick here for information on saphenous vein blood sampling in the guinea pigClick here for information on cardiac puncture blood sampling in the guinea pigClick here for information on schedule 1 stunning followed by decapitation for blood sampling in the guinea pig