To assess whether refinements to laboratory procedures do indeed improve animal welfare, it is essential that we use scientifically validated measures of welfare. A key determinant of welfare is an animal's affective (emotional) state. Subjective emotions cannot be measured directly so proxy indicators are used instead.
Existing indicators have significant shortcomings and we have therefore developed a new approach, inspired by the links between emotion and cognition observed in humans, and grounded in theoretical models of decision-making. The core hypothesis is that individuals in negative affective states judge ambiguous stimuli negatively compared to happier individuals. We have developed a task to test for such 'cognitive biases' in animals and have found, in several species, that putative affective state is related to judgement bias as predicted.
However, our tests are time-consuming and have not yet been extended to mice, the most commonly used laboratory species. We will therefore develop an automated version of our generic judgement bias task for laboratory rodents. We will use environmental and pharmacological manipulations of affective state to validate our new test. We will investigate whether judgement bias size relates to intensity of the induced state (using dose-response studies), and hence whether our approach can quantify emotional intensity. We will also investigate whether the test can detect the cumulative effects of long-term experience on affective state (e.g. by comparing animals kept in enriched and standard housing conditions).
An industrial partner has expressed interest in further implementing the test that we develop for widespread use. The study will also produce computational models of how affective states change in response to external events. These may provide the basis for modelling the effects of experimental treatments on an animal's affective state, hence facilitating better and more humane planning of studies.
Paul ES et al. (2020). Towards a comparative science of emotion: Affect and consciousness in humans and animals. Neuroscience and biobehavioral reviews 108:749-770. doi: 10.1016/j.neubiorev.2019.11.014
Jones S et al. (2018). Assessing animal affect: an automated and self-initiated judgement bias task based on natural investigative behaviour. Sci Rep. 8(1):12400. doi: 10.1038/s41598-018-30571-x
Paul ES and Mendl MT (2018). Animal emotion: Descriptive and prescriptive definitions and their implications for a comparative perspective. Applied Animal Behaviour Science doi: 10.1016/j.applanim.2018.01.008
Bach D and Dayan P (2017). Algorithms for survival: a comparative perspective on emotions. Nature Reviews Neuroscience 18(5):311-319. doi: 10.1038/nrn.2017.35
Jones S et al. (2017). Pavlovian influences on learning differ between rats and mice in a counter-balanced Go/NoGo judgement bias task. Behavioural Brain Research 331:214-224. doi: 10.1016/j.bbr.2017.05.044
Mendl M et al. (2017). Animal Welfare Science, in Call J (ed.) APA Handbook of Comparative Psychology. American Psychological Association Inc. ISBN: 9781433823480
Iigaya K et al. (2016). Cognitive Bias in Ambiguity Judgements: Using Computational Models to Dissect the Effects of Mild Mood Manipulation in Humans. PloS ONE 11(11):e0165840. doi: 10.1371/journal.pone.0165840
Paul ES and Mendl MT. (2016) If insects have phenomenal consciousness, could they suffer? Animal Sentience 9(16).
Trimmer PC et al. (2013). On the evolution and optimality of mood states. Behavioral Sciences 3:501–521. doi: 10.3390/bs3030501
- Engagement Activities: Measuring animal emotion workshop - impressions and Storify
- Poster: Developing an automated test of laboratory rodent affect and welfare
- Resources: Rodent Welfare Hub
Principal investigatorProfessor Michael Mendl
InstitutionUniversity of Bristol
Co-InvestigatorProfessor Peter Dayan
Dr Emma Robinson
Dr Elizabeth Paul