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NC3Rs: National Centre for the Replacement Refinement & Reduction of Animals in Research
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Oct 21, 2013 ... Intraperitoneal injection of Pentobarbitone causes peritoneal irritation and potentially pain due to its alkalinity. Physical methods such ...

Oct 29, 2014 ... The most frequently used are pronuclear injection, embryonic stem cells, chemical mutagenesis and the new genome-editing techniques such as ...