Skip to main content

New funding scheme: £4M available for proposals to establish infrastructure needed to accelerate use of non-animal methods. Apply now.

NC3Rs | 20 Years: Pioneering Better Science

Rat playpens

The positive impact playpens have on laboratory rat welfare, and guidance on how to set one up in your institution.


A playpen, or playroom, is a designated enriched space that is usually provided as an addition to the standard housing of animals. Access to a playpen has a positive impact on research animal health and wellbeing, which can consequently benefit animal technicians and researchers. This resource outlines these benefits and provides practical advice, focusing on playpens for rats. Some of the general principles and literature may also be relevant to other species.

The benefits of playpens

Playpens provide captive animals with additional space and the chance to interact with a wider range of enrichment than what can usually be provided within standard caging. Playpens create opportunities to:

Improve overall wellbeing and scientific outcomes

Facilitating animals to perform natural, healthy behaviours is beneficial to their physiological and psychological wellbeing. Within a research environment, traditional housing often restrict what behaviours can be performed.

Small cages with only basic enrichment limit the ability of animals to exercise and engage with their environment. When behaviour is constrained in this way animals can become chronically unfit, overweight and understimulated, resulting in abnormal physiology and behaviour. Ultimately, data obtained from these animals could lead to unreliable conclusions, regardless of the area of study [1,2].

Access to a playpen provides a solution to the welfare challenges associated with research-animal housing. Rats in enriched environments display a wider range of behaviours than is possible in traditional caging, including vertical stretching, hopping, burrowing and climbing [3,4]. In addition to opportunities for exercise and wider behavioural expression, welfare benefits are also indicated by the preference of rats for more complex, enriched environments [see 5 for an overview].

A recent study has shown that rats produced a higher number of 50 kHz vocalisations during sessions in a ball pit and playpen compared with control conditions [6]; these 50 kHz calls are within a range repeatedly shown to be associated with positive affect (mood) and welfare [7, 8]. Furthermore, playpen time mitigated the negative effects of an aversive drug treatment in these rats, suggesting that the use of playpens could reduce the negative impacts of some aversive procedures.


Provide additional socialisation

Social interactions are crucial for the development and wellbeing of social species, such as rats [9].

However, it is not always possible to house research animals in their preferred group size, and in exceptional circumstances it may be necessary to singly house rats during a study, or for breeding purposes. A lack of social interaction can have a major impact on rat welfare and there is no true substitute for being able to engage with other rats.

The social mixing of rats within a playpen provides an opportunity for them to interact in a positive way. Even if the rats do not directly interact with each other (e.g. by playing or huddling), they will still be indirectly engaging by smelling the scents and hearing the vocalisations of other animals.  


Kate Shenton (AstraZeneca) spoke about her experiences providing rats with additional opportunities for exercise and socialisation at IAT Congress 2017: playtime for rats.

Carina Christoffersen, an Animal Welfare Technician at Ellegaard Göttingen Minipigs, Denmark, noted that access to a playroom facilitated the formation of stable social groups in young pigs. Carina was featured as a 3Rs Champion in Tech3Rs, our newsletter for animal technicians.

Reduce handling-associated stress

Handling an uncooperative animal can be stressful, and even dangerous, for both the animal and the handler. There can be additional stress for the animal if they associate being handled with negative experiences (e.g. aversive scientific procedures).

Playpens provide an opportunity for regular positive human-rat interactions, which makes rats calmer and easier to handle [5] and can even lead to rats actively seeking out interactions with humans. Positive human-rat interactions are important for both animal and staff wellbeing [5,10]


Habituate animals to new objects

Playpens can be used to introduce animals to novel equipment, such as transport containers or scientific tools, before they are exposed to them in day-to-day use.

Novel objects are placed into the playpen, along with a range of enrichment items, allowing the rats to engage with the objects on their own terms. Rats are motivated to explore and spending time in enriched spaces can accelerate their habituation to novelty [11]. Treats can be placed in or around the equipment to encourage the animals to investigate and to build a positive association.


We spoke to Sarah Taylor, an animal technician at the University of Cambridge, about using playrooms to habituate rats to transport boxes. Sarah was featured as a 3Rs Champion in Tech3Rs our newsletter for animal technicians.
A perforated, cardboard transport carrier sits on its side in a playpen. The carrier is filled with strips of bedding material and surrounded by brightly coloured balls.
Rat transport box. Image credit: Cambridge University.

Improve job satisfaction for animal technicians

There can be a cost of caring for research animals, which can lead to laboratory animal professionals experiencing compassion fatigue [10]. Being able to provide good environmental enrichment is a factor related to improved job satisfaction for animal technicians [10].

Playpens add an enjoyable aspect to the role of animal technicians, allowing them to express creativity, take ownership of an animal welfare project and experience positive human-animal interaction.


The NA3RsC has created a resource for research animal professionals on compassion fatigue.

Dr Megan LaFollette discussed compassion fatigue research and improving staff wellbeing in an animal research setting and how it can affect the mental wellbeing of animal care staff in this joint NC3Rs and NA3RsC webinar.

Recycle old equipment

Animal facilities can generate a lot of waste and budgets can be tight. Playpens provide an opportunity to recycle items that may otherwise be discarded. For example, an old rabbit pen with some damage is not suitable for use as a permanent enclosure, but it could be repurposed as a rat playpen. Packaging materials, such as old glove boxes, can be an appealing place for rats for to huddle, or they may just enjoy shredding the cardboard.

It is important to ensure that repurposed materials will be safe for the rats, but it is possible to create a playpen using entirely recycled items.  


We spoke to Joe Peploe and Sarah Taylor, animal technicians at the University of Cambridge, who both use repurposed materials to create enrichment for their animals. Joe and Sarah were featured as 3Rs Champions in Tech3Rs our newsletter for animal technicians.

Setting up a rat playpen

Key features of rat playpens

  • Larger than standard housing; adequate space to create a complex environment.
  • Vertical height of >30 cm; enough room to rear to full height.
  • A range of enrichment items not found within the home cage.
  • Social enrichment i.e., the opportunity to engage with other rats.

Tips for setting up a rat playpen

  • Discuss your plans with colleagues. You may wish to consider the barriers to adoption and ways to overcome these that were outlined in this IAT Congress workshop summary.
  • Source an enclosure to use as a playpen. Rat playpens can be built by repurposing old cages, for example, Xenopus tanks, rabbit cages, diet bins and marmoset cages. The playpen needs to have vertical and horizontal space to allow the rats to run, hop, rear to their full height and engage in social behaviours.
  • Source appropriate items for your playpen, ensuring that the pen itself and the enrichment items are safe and clean. Consult your NACWO or veterinarian if you have any doubts about suitability.
  • To keep down costs you can furnish the playpen with enrichment items from within the facility, for example empty glove boxes, paper towel rolls, bins filled with nesting material, dishes of shallow water.
  • Your aim is to create a complex environment that engages the senses and facilitates a range of natural, healthy behaviours. Enrichment that meets these criteria includes treats scattered within substrate, scented gnaw sticks, soil for burrowing and ladders for climbing. Consider creating your own puzzle feeders to cognitively engage the rats.
  • Consider the biosecurity of your unit and what is possible within these constraints. This will determine the groupings of rats within a playpen session and the cleaning schedule.
  • Monitor playpen use to ensure safety and suitability. You may wish to carry out a small study to see whether access to a playpen improves welfare.


For more practical advice on setting up rat playpens see:

Kate Shenton’s (AstraZeneca) practical strategies for providing rats with a complex environment.

The NC3Rs webinar: Rat playpens for improved welfare.

A tall cage which has been used to create a rat playpen. There are three different tiers with ladders to allow the rats to climb up between each one. A number of white rats can be seen moving between the different levels. Enrichment and play objects are available on each level including an cardboard box that used to hold laboratory gloves, coloured balls, tubes, a wheel and a raised platform on the top level with some bedding material.
An example of a rat playpen. Image courtesy of the Preclinical Research Facility, University of Leicester.

Rat playpen FAQs

Playpens for rats webinar

Dr Jessica Eddy of the NC3Rs presents an overview of playpens and tips for setting them up, and Kirsty Watson, a Senior Animal Technician, outlines how rat playpens were introduced at the Institute of Ophthalmology at University College London and the positive results staff saw.




  1. ​​​​Poole T (1997). Happy animals make good science. Laboratory Animals 31, 116-124. doi: 10.1258/002367797780600198
  2. Martin B et al. (2010). "Control" laboratory rodents are metabolically morbid: Why it matters. PNAS 107 (14):6127-6133. doi: 10.1073/pnas.0912955107
  3. Berdoy M (2002) The Laboratory Rat: A Natural History. Film. 27 minutes. The film can be viewed at
  4. Makowska IJ and Weary DM (2016). The importance of burrowing, climbing and standing upright for laboratory rats. Royal Society Open Science 3: 160136. doi: 10.1098/rsos.160136
  5. Makowska IJ and Weary DM (2019). A good life for laboratory rodents?. ILAR journal 60(3): 373-388. doi: 10.1093/ilar/ilaa001
  6. Hinchcliffe JK et al. (2021). The use of ball pits and playpens in laboratory Lister Hooded male rats induces ultrasonic vocalisations indicating a more positive affective state and can reduce the welfare impacts of aversive procedures. Laboratory Animals January 2022. doi: 10.1177/00236772211065920
  7. Burke CJ et al. (2017) Specific 50-kHz vocalizations are tightly linked to particular types of behavior in juvenile rats anticipating play. PloS One 12(5). doi: 10.1371/0175841  
  8. Hinchcliffe JK et al. (2020). Rat 50 kHz calls reflect graded tickling-induced positive emotion. Current Biology 30(18). doi: 10.1016/j.cub.2020.08.038
  9. Berdoy M and Drickamer LL (2007). Comparative Social Organisation and Life History of Rattus and Mus, pp 380-392. In: Rodent Societies: An ecological and evolutionary perspective (Eds. Wolff JO and Sherman PW). 1st edition. Univeristy of Chigago Press.
  10. LaFollette MR et al. (2020). Laboratory animal welfare meets human welfare: a cross-sectional study of professional quality of life, including compassion fatigue in laboratory animal personnel. Frontiers in veterinary science 7, 114. doi: 10.3389/fvets.2020.00114
  11. Modlinska K et al. (2019). The impact of changeability of enriched environment on exploration in rats. Behavioural processes 164: 78-85. doi: 10.1016/j.beproc.2019.04.015
  12. Brydges NM et al. (2011). Environmental enrichment induces optimistic cognitive bias in rats. Animal Behaviour 81(1): 169-175. doi: 10.1016/j.anbehav.2010.09.030
  13. Wallace DJ et al. (2013). Rats maintain an overhead binocular field at the expense of constant fusion. Nature 498(7452): 65-69. doi: 10.1038/nature12153
  14. Farnworth B et al. (2020). Antipredator responses of ship rats to visual stimuli: combining unimodal predation cues generates risk avoidance. Animal Behaviour 168, 149-157. doi: 10.1016/j.anbehav.2020.08.007
  15. Ratuski AS et al. (2021). Using approach latency and anticipatory behaviour to assess whether voluntary playpen access is rewarding to laboratory mice. Scientific reports 11(1): 1-13. doi: /10.1038/s41598-021-98356-3
  16. Pritchett-Corning KR (2019). Environmental complexity and research outcomes. ILAR journal, 60(2): 239-251. doi: 10.1093/ilar/ilaa007